Live demo · synthetic standard-curve assay

This is what “understand the experiment first” looks like.

No signup. AssayPilot detected an explicit standard-curve workflow, fitted the standards, back-calculated unknowns, applied the supplied dilution factor and kept QC visible.

Model4PL
R²1.000
Unknowns2

QC

PASSCalibration fit

4pl · R² = 1.000

PASSReplicates

0 sample group(s) above 20% CV

Why this analysis?

Standards, concentrations and assay signal were detected. Auto-selected 4pl by lower AICc (-547.92 vs -10.49).

Calibration model choice is based on the supplied standards; inspect residuals and assay-specific validation before publication.

Calculated figure

Not a screenshot.

This plot was generated by the same deterministic engine used for uploaded runs.

AssayPilot standard curve demo
Now try your own ugly export